Part:BBa_M36546:Design

apFAB47 Promoter with DNA binding sequence from CRP

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 25
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 25
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 25
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Between the -35 box and the -10 box, we deleted the sequence from apFAB47 promoter and replaced it with the binding sequence that CRP will bind to.

Source

BioFab http://io.biofab.org/services/studio/dac/ http://www.uniprot.org/uniprot/P0ACJ8